Bouazzaoui, Abdellatif and Kreutz, Marina and Eisert, Veronika and Dinauer, Norbert and Heinzelmann, Anna and Hallenberger, Sabine and Strayle, Jochen and Walker, Russel and Ruebsamen-Waigmann, Helga and Andreesen, Reinhard and von Briesen, Hagen (2006) Stimulated trans-acting factor of 50 kDa (Staf50) inhibits HIV-1 replication in human monocyte-derived macrophages. VIROLOGY, 356 (1-2). pp. 79-94. ISSN 0042-6822,
Full text not available from this repository. (Request a copy)Abstract
In order to identify cellular genes which interfere with HIV-1 replication in monocyte-derived macrophages (MAC), cells were stimulated with interferon (IFN) or lipopolysaccharide (LPS) leading to a pronounced inhibition of HIV-1 infection in these cells, and the resulting gene expression was analyzed. Using the microarray technology we identified a gene named Stimulated Trans-Acting Factor of 50 kDa (Staf50), which is known to repress the activity of the HIV-1 LTR. Analysis of the Staf50 expression by real-time PCR showed an overexpression in IFN alpha (up to 20-fold) and LPS (up to 10-fold)-stimulated MAC as well as in infected cells (up to 3-fold). For stable overexpression, 293 T cells and primary macrophages were transduced with Staf50-IRES-GFP bicistronic pseudotype viruses. After transduction, 293 T CD4/CCR5 and MAC were infected with HIV-1, and virus replication was monitored by p24 ELISA. Overexpression of Staf50 inhibited the HIV-1 infection between 50% and 90% in 293 T CD4/CCR5 as well as in MAC. Our findings suggest that host genetic effects in combination with viral properties determine the susceptibility of an appropriate target cell for HIV-1 infection as well as the replication potential of the virus in the cell resulting in an overall productive infection. (c) 2006 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN-IMMUNODEFICIENCY-VIRUS; VITAMIN-A SUPPLEMENTS; IN-SITU HYBRIDIZATION; VIVO GENE DELIVERY; TYPE-1 REPLICATION; TUMOR SUPPRESSION; VIF PROTEIN; EXPRESSION; CELLS; INFECTION; monocytes/macrophages; HIV replication; lipopolysaccharide; interferon; gene profiling; Staf50 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Jan 2021 09:03 |
| Last Modified: | 18 Jan 2021 09:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33742 |
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