Banzi, Manuela and Aguiari, Gianluca and Trimi, Viky and Mangolini, Alessandra and Pinton, Paolo and Witzgall, Ralph and Rizzuto, Rosario and del Senno, Laura (2006) Polycystin-1 promotes PKC alpha-mediated NF-kappa B activation in kidney cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 350 (2). pp. 257-262. ISSN 0006-291X, 1090-2104
Full text not available from this repository. (Request a copy)Abstract
Polycystin-1 (PC1), the PKD1 gene product, is a membrane receptor which regulates many cell functions, including cell proliferation and apoptosis, both typically increased in cyst lining cells in autosomal dominant polycystic kidney disease. Here we show that PC] upregulates the NF-kappa B signalling pathway in kidney cells to prevent cell death. Human embryonic kidney cell lines (HEK293(CTT)), stably expressing a PC1 cytoplasmic terminal tail (CTT), presented increased NF-kappa B nuclear levels and NF-kappa B-mediated luciferase promoter activity. This, consistently, was reduced in HEK293 cells in which the endogenous PC1 was depleted by RNA interference. CTT-dependent NF-kappa B promoter activation was mediated by PKC alpha because it was blocked by its specific inhibitor Ro-320432. Furthermore, it was observed that apoptosis, which was increased in PC1-depleted cells, was reduced in HEK293CTT cells and in porcine kidney LtTA cells expressing a doxycycline-regulated CTT. Staurosporine, a PKC inhibitor, and parthenolide, a NF-kappa B inhibitor, significantly reduced the CTT-dependent antiapoptotic effect. These data reveal, therefore, a novel pathway by which polycystin-1 activates a PKC alpha-mediated NF-kappa B signalling and cell survival. (c) 2006 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | APOPTOSIS; PROTEIN; MECHANOSENSATION; PROLIFERATION; RELEASE; OUABAIN; CYCLE; polycystin-1; NF-kappa B; promoter activity; siRNA; apoptosis; PKC alpha |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Ralph Witzgall |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Jan 2021 09:23 |
| Last Modified: | 19 Jan 2021 09:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33755 |
Actions (login required)
 |
View Item |
