Knerr, Robert and Weiser, Barbara and Drotleff, Sigrid and Steinem, Claudia and Goepferich, Achim (2006) Measuring cell adhesion on RGD-modified, self-assembled PEG monolayers using the quartz crystal microbalance technique. MACROMOLECULAR BIOSCIENCE, 6 (10). pp. 827-838. ISSN 1616-5187,
Full text not available from this repository. (Request a copy)Abstract
In this study, the suitability of a flow-through quartz. crystal microbalance system for the detection of the adhesion of rMSCs and 3T3-L1 fibroblasts on different surfaces is demonstrated. Frequency shifts for rMSCs of -6.7 mHz center dot cell(-1) and -2.0 mHz center dot cell(-1) for 3T3-L1 cells could be detected on non-modified gold sensors, revealing that the frequency shift per cell is comparable to that of a static setup. Modifying the sensor surface with SAMs of thioalkylated omega-amine-terminated PEG derivatives led to cell-adhesion-resistant surfaces. Total frequency shifts of only -20 +/- 7 Hz showed that protein adsorption was also significantly reduced. Attaching 35 pmol center dot mm(-2) of the GRGDS cell adhesion motif to the SAMs induced specific cell adhesion due to RGD-integrin interactions; the resonance frequency dropped by 3.4 mHz center dot cell(-1). Furthermore, 1 the kinetics of cell detachment could be determined. The corresponding processes were completed after 10 min for trypsin, and not before 90 min with GRGDS. Moreover, the detectability of cell adhesion was shown to increase after the addition of manganese cations. The total decrease in the resonance frequency was almost 80 Hz in the presence of Mn2+ (6.4 mHz center dot cell(-1)).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SURFACE-PLASMON RESONANCE; MAMMALIAN-CELLS; ENDOTHELIAL-CELLS; INTEGRIN FUNCTION; ATTACHMENT; PROTEIN; BIOSENSOR; POLYMERS; GROWTH; FILMS; biomaterials; biomimetic; cell adhesion; poly(ethylene glycol); self-assembly |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jan 2021 09:19 |
| Last Modified: | 20 Jan 2021 09:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33869 |
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