Rivera, Francisco J. and Sierralta, Walter D. and Minguell, Jose J. and Aigner, Ludwig (2006) Adult hippocampus derived soluble factors induce a neuronal-like phenotype in mesenchymal stem cells. NEUROSCIENCE LETTERS, 406 (1-2). pp. 49-54. ISSN 0304-3940, 1872-7972
Full text not available from this repository. (Request a copy)Abstract
Bone marrow-derived mesenchymal stem cells (MSCs) are not restricted in their differentiation fate to cells of the mesenchymal lineage. They acquire a neural phenotype in vitro and in vivo after transplantation in the central nervous system. Here we investigated whether soluble factors derived from different brain regions are sufficient to induce a neuronal phenotype in MSCs. We incubated bone marrow-derived MSCs in conditioned medium (CM) derived from adult hippocampus (HCM), cortex (CoCM) or cerebellum (CeCM) and analyzed the cellular morphology and the expression of neuronal and glial markers. In contrast to muscle derived conditioned medium, which served as control, conditioned medium derived from the different brain regions induced a neuronal morphology and the expression of the neuronal markers GAP-43 and neurofilaments in MSCs. Hippocampus derived conditioned medium had the strongest activity. It was independent of NGF or BDNF; and it was restricted to the neuronal differentiation fate, since no induction of the astroglial marker GFAP was observed. The work indicates that soluble factors present in the brain are sufficient to induce a neuronal phenotype in MSCs. (c) 2006 Elsevier Ireland Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MARROW STROMAL CELLS; BONE-MARROW; IN-VITRO; DIFFERENTIATION; RATS; TRANSPLANTATION; ASTROCYTES; RECOVERY; PROTEIN; BRAINS; differentiation; stem cells; hippocampus; neuron |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jan 2021 11:03 |
| Last Modified: | 20 Jan 2021 11:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33896 |
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