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Full text not available from this repository. (Request a copy)Abstract
This paper describes the developments, role and contributions of the NMR spectroscopy groups in the Structural Proteomics In Europe ( SPINE) consortium. Focusing on the development of high- throughput ( HTP) pipelines for NMR structure determinations of proteins, all aspects from sample preparation, data acquisition, data processing, data analysis to structure determination have been improved with respect to sensitivity, automation, speed, robustness and validation. Specific highlights are protonless C-13-direct detection methods and inferential structure determinations ( ISD). In addition to technological improvements, these methods have been applied to deliver over 60 NMR structures of proteins, among which are five that failed to crystallize. The inclusion of NMR spectroscopy in structural proteomics pipelines improves the success rate for protein structure determinations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-BACKBONE ASSIGNMENT; NUCLEOTIDE EXCISION-REPAIR; COPPER TRAFFICKING PROTEIN; AUTOMATED NOE ASSIGNMENT; HUMAN BETA-PARVALBUMIN; CYTOCHROME-C-OXIDASE; SOLID-STATE NMR; PARAMAGNETIC PROTEINS; EFFICIENT STRATEGY; ALPHA-PARVALBUMIN; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Jan 2021 11:09 |
| Last Modified: | 20 Jan 2021 11:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33897 |
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