Borisov, Sergey M. and Neurauter, Gerhard and Schroeder, Claudia and Klimant, Ingo and Wolfbeis, Otto S. (2006) Modified dual lifetime referencing method for simultaneous optical determination and sensing of two analytes. APPLIED SPECTROSCOPY, 60 (10). pp. 1167-1173. ISSN 0003-7028,
Full text not available from this repository. (Request a copy)Abstract
Simultaneous fluorometric sensing of two analytes becomes possible using a modified dual lifetime referencing (m-DLR) method. In this scheme, two luminescent indicators are needed that have overlapping absorption and emission spectra but largely different decay times. They are excited by a single light source, and both emissions are measured simultaneously. In the frequency domain m-DLR method, the phase of the short-lived fluorescence of a first indicator is referenced against that of the long-lived luminescence of the second indicator. The analytical information is obtained by measurement of the phase shifts at two modulation frequencies. The method is demonstrated to work for the case of dually sensing oxygen and carbon dioxide. It benefits from simple instrumentation and optical setup. The approach is perceived to be of wide applicability. Examples include (1) analysis of two luminescent analytes, (2) analytical determinations that make use of two probes, and (3) sensing of two species such as carbon dioxide and oxygen (as demonstrated here), or oxygen and chlorophyll, provided the luminophores meet the condition of having largely different decay times and overlapping absorption and emission spectra.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CARBON-DIOXIDE SENSOR; RESONANCE ENERGY-TRANSFER; METAL-COMPLEXES; CHEMICAL SENSORS; DISSOLVED-OXYGEN; PH; TEMPERATURE; PHOTOPHYSICS; TECHNOLOGY; FIBER; sensing; fluorescence; luminescence lifetime; signal referencing |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jan 2021 06:56 |
| Last Modified: | 25 Jan 2021 06:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33907 |
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