Obermeier, Florian and Hausmann, Martin and Kellermeier, Silvia and Kiessling, Stefan and Strauch, Ulrike G. and Duitman, Erwin and Bulfone-Paus, Silvia and Herfarth, Hans and Bock, Juergen and Dunger, Nadja and Stoeck, Michael and Schoelmerich, Juergen and Falk, Werner and Rogler, Gerhard (2006) IL-15 protects intestinal epithelial cells. EUROPEAN JOURNAL OF IMMUNOLOGY, 36 (10). pp. 2691-2699. ISSN 0014-2980,
Full text not available from this repository. (Request a copy)Abstract
IL-15, a T-cell growth factor, has been shown to be increased in inflammatory bowel disease (IBD). It has been suggested that neutralization of IL-15 could protect from T cell-dependent autoimmune inflammation. On the other hand, an anti-apoptotic effect of IL-15 has been demonstrated in kidney epithelial cells during nephritis. We therefore tested the role of IL-15 in two different experimental models of colitis in vivo, and in models of intestinal epithelial cell (IEC) apoptosis in vitro. IL-15 blockade in chronic dextran sulphate sodium-induced colitis resulted in aggravation of the disease with a significantly 2.1-fold increased epithelial damage score compared to controls. TUNEL staining clearly revealed increased apoptosis. IL-6, TNF and IFN-gamma secretion by mesenteric lymph node cells were increased. In the T cell-dependent SCID transfer model of colitis IL-15 neutralization reduced the inflammatory infiltration and proinflammatory cytokine production. Despite that, the intestinal epithelial damage was not reduced. In vitro, IL-15 pre-incubation prevented up to 75% of CH11 antibody-induced apoptosis in SW-480 cells and reduced caspase-3 activity. According to this, endogenously produced IL-15 in chronic colitis does not only act as a proinflammatory cytokine but has at the same time the potential to reduce mucosal damage by preventing IEC apoptosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY-BOWEL-DISEASE; DEXTRAN SULFATE SODIUM; T-CELLS; RHEUMATOID-ARTHRITIS; ULCERATIVE-COLITIS; INTERLEUKIN 15; INTRAEPITHELIAL LYMPHOCYTES; IN-VIVO; CYTOKINE PRODUCTION; SURVIVAL FACTOR; apoptosis; colitis; IL-15; intestinal epithelial cells; mucosa |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jan 2021 07:24 |
| Last Modified: | 25 Jan 2021 07:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33917 |
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