Naether, Daniela J. and Rachel, Reinhard and Wanner, Gerhard and Wirth, Reinhard (2006) Flagella of Pyrococcus furiosus: Multifunctional organelles, made for swimming, adhesion to various surfaces, and cell-cell contacts. JOURNAL OF BACTERIOLOGY, 188 (19). pp. 6915-6923. ISSN 0021-9193,
Full text not available from this repository. (Request a copy)Abstract
Pyrococcus furiosus ("rushing fireball") was named for the ability of this archaeal coccus to rapidly swim at its optimal growth temperature, around 100 degrees C. Early electron microscopic studies identified up to 50 cell surface appendages originating from one pole of the coccus, which have been called flagella. We have analyzed these putative motility organelles and found them to be composed primarily (> 95%) of a glycoprotein that is homologous to flagellins from other archaea. Using various electron microscopic techniques, we found that these flagella can aggregate into cable-like structures, forming cell-cell connections between ca. 5% of all cells during stationary growth phase. P. furiosus cells could adhere via their flagella to carbon-coated gold grids used for electron microscopic analyses, to sand grains collected from the original habitat (Porto di Levante, Vulcano, Italy), and to various other surfaces. P. furiosus grew on surfaces in biofilm-like structures, forming microcolonies with cells interconnected by flagella and adhering to the solid supports. Therefore, we concluded that P. furiosus probably uses flagella for swimming but that the cell surface appendages also enable this archaeon to form cable-like cell-cell connections and to adhere to solid surfaces.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HYPERTHERMOPHILIC ARCHAEA; ELECTRON CRYOMICROSCOPY; METHANOCOCCUS-VOLTAE; ANGSTROM RESOLUTION; MOTILITY STRUCTURE; SP-NOV; FILAMENTS; SALMONELLA; ROTATION; ARCHAEBACTERIUM; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Wirth Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jan 2021 09:26 |
| Last Modified: | 25 Jan 2021 09:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33947 |
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