Schiessl, Ina Maria and Castrop, Hayo (2016) Deep insights: intravital imaging with two-photon microscopy. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 468 (9). pp. 1505-1516. ISSN 0031-6768, 1432-2013
Full text not available from this repository. (Request a copy)Abstract
Intravital multiphoton microscopy is widely used to assess the structure and function of organs in live animals. Although different tissues vary in their accessibility for intravital multiphoton imaging, considerable progress has been made in the imaging quality of all tissues due to substantial technical improvements in the relevant imaging components, such as optics, excitation laser, detectors, and signal analysis software. In this review, we provide an overview of the technical background of intravital multiphoton microscopy. Then, we note a few seminal findings that were made through the use of multiphoton microscopy. Finally, we address the technical limitations of the method and provide an outlook for how these limitations may be overcome through future technical developments.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACUTE KIDNEY INJURY; VIVO MULTIPHOTON MICROSCOPY; SINGLE-CELL LEVEL; IN-VIVO; FLUORESCENCE MICROSCOPY; LEUKOCYTE RECRUITMENT; EXCITATION MICROSCOPY; CYCLIC-NUCLEOTIDES; REACTIVE GLIOSIS; PODOCYTE INJURY; Intravital imaging; Multiphoton microscopy; Labeling strategies |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Wolf Hayo Castrop |
| Depositing User: | Petra Gürster |
| Date Deposited: | 28 Aug 2020 10:53 |
| Last Modified: | 28 Aug 2020 10:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3397 |
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