Kessler, Alexandra and Sezonov, Guennadi and Guijarro, J. Inaki and Desnoues, Nicole and Rose, Thierry and Delepierre, Muriel and Bell, Stephen D. and Prangishvili, David (2006) A novel archaeal regulatory protein, Sta1, activates transcription from viral promoters. NUCLEIC ACIDS RESEARCH, 34 (17). pp. 4837-4845. ISSN 0305-1048,
Full text not available from this repository. (Request a copy)Abstract
While studying gene expression of the rudivirus SIRV1 in cells of its host, the hyperthermophilic crenarchaeon Sulfolobus, a novel archaeal transcriptional regulator was isolated. The 14 kDa protein, termed Sulfolobus transcription activator 1, Sta1, is encoded on the host chromosome. Its activating effect on transcription initiation from viral promoters was demonstrated in in vitro transcription experiments using a reconstituted host system containing the RNA polymerase, TATA-binding protein (TBP) and transcription factor B (TFB). Most pronounced activation was observed at low concentrations of either of the two transcription factors, TBP or TFB. Sta1 was able to bind viral promoters independently of any component of the host pre-initiation complex. Two binding sites were revealed by footprinting, one located in the core promoter region and the second similar to 30 bp upstream of it. Comparative modeling, NMR and circular dichroism of Sta1 indicated that the protein contained a winged helix-turn-helix motif, most probably involved in DNA binding. This strategy of the archaeal virus to co-opt a host cell regulator to promote transcription of its genes resembles eukaryal virus-host relationships.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-FREE TRANSCRIPTION; TATA-BINDING PROTEIN; PYROCOCCUS-FURIOSUS; RNA-POLYMERASE; IN-VITRO; SULFOLOBUS-ACIDOCALDARIUS; METHANOCOCCUS-VANNIELII; ANTIBIOTIC-RESISTANCE; SECONDARY STRUCTURE; CIRCULAR-DICHROISM; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Jan 2021 10:12 |
| Last Modified: | 25 Jan 2021 10:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33979 |
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