Rivera, Francisco T. and Couillard-Despres, Sebastien and Pedre, Xiomara and Ploetz, Sonja and Caioni, Massimiliano and Lois, Carlos and Bogdahn, Ulrich and Aigner, Ludwig (2006) Mesenchymal stem cells instruct oligodendrogenic fate decision on adult neural stem cells. STEM CELLS, 24 (10). pp. 2209-2219. ISSN 1066-5099,
Full text not available from this repository. (Request a copy)Abstract
Adult stem cells reside in different tissues and organs of the adult organism. Among these cells are MSCs that are located in the adult bone marrow and NSCs that exist in the adult central nervous system (CNS). In transplantation experiments, MSCs demonstrated neuroprotective and neuro-regenerative effects that were associated with functional improvements. The underlying mechanisms are largely unidentified. Here, we reveal that the interactions between adult MSCs and NSCs, mediated by soluble factors, induce oligodendrogenic fate decision in NSCs at the expense of astrogenesis. This was demonstrated (a) by an increase in the percentage of cells expressing the oligodendrocyte markers GaIC and myelin basic protein, (b) by a reduction in the percentage of glial fibrillary acidic protein (GFAP)-expressing cells, and (c) by the expression pattern of cell fate determinants specific for oligodendrogenic differentiation. Thus, it involved enhanced expression of the oligodendrogenic transcription factors Olig1, Olig2, and Nkx2.2 and diminished expression of Id2, an inhibitor of oligodendrogenic differentiation. Results of (a) 5-bromo-2'-deoxyuridine pulse-labeling of cells, (b) cell fate analysis, and (c) cell death/survival analysis suggested an inductive mechanism and excluded a selection process. A candidate factor screen excluded a number of growth factors, cytokines, and neurotrophins that have previously been shown to influence neurogenesis and neural differentiation from the oligodendrogenic activity derived from the MSCs. This work might have major implications for the development of future transplantation strategies for the treatment of degenerative diseases in the CNS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MARROW STROMAL CELLS; CENTRAL-NERVOUS-SYSTEM; INJURED SPINAL-CORD; LOOP-HELIX PROTEIN; TRANSCRIPTION FACTORS; PROGENITOR CELLS; IN-VITRO; DIFFERENTIATION; EXPRESSION; GROWTH; adult stem cells; transcription factor; somatic stem cells; real-time reverse transcription-polymerase chain reaction oligodendrocytes; enhanced green fluorescent protein; differentiation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Jan 2021 15:50 |
| Last Modified: | 26 Jan 2021 15:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/33999 |
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