Weissinger, E. M. and Human, C. and Metzger, J. and Hambach, L. and Wolf, D. and Greinix, H. T. and Dickinson, A. M. and Mullen, W. and Jonigk, D. and Kuzmina, Z. and Kreipe, H. and Schweier, P. and Bohm, O. and Turuchanow, I. and Ihlenburg-Schwarz, D. and Raad, J. and Durban, A. and Schiemann, M. and Konecke, C. and Diedrich, H. and Holler, E. and Beutel, G. and Krauter, J. and Ganser, A. and Stadler, M. (2017) The proteome pattern cGvHD_MS14 allows early and accurate prediction of chronic GvHD after allogeneic stem cell transplantation. LEUKEMIA, 31 (3). pp. 654-662. ISSN 0887-6924, 1476-5551
Full text not available from this repository. (Request a copy)Abstract
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) may be curative, but is associated with significant morbidity and mortality. Chronic graft-versus-host disease (cGvHD), characterized by inflammation and fibrosis of multiple target organs, considerably contributes to the morbidity and mortality even years after allo-HSCT. Diagnosis of cGvHD is based on clinical features and histology of biopsies. Here, we report the generation of a urinary cGvHD-specific proteome-pattern (cGvHD_MS14) established by capillary electrophoresis-mass spectrometry to predict onset and severity of cGvHD as an unbiased laboratory test. cGvHD_MS14 was evaluated on samples from 412 patients collected prospectively in four transplant centers. Sensitivity and specificity was 84 and 76% by cGvHD_MS14 classification. Sensitivity further increased to 93% by combination of cGvHD_MS14 with relevant clinical variables to a logistic regression model. cGvHD was predicted up to 55 days prior to clinical diagnosis. Acute GvHD is not recognized by cGvHD_MS14. cGvHD_MS14 consists of 14 differentially excreted peptides, six of those have been sequenced to date and are fragments from thymosin beta-4, eukaryotic translation initiation factor 4 gamma 2, fibrinogen beta-chain or collagens. In conclusion, the cGvHD_MS14-pattern allows early, highly sensitive and specific prediction of cGvHD as an independent diagnostic criterion of clinical diagnosis potentially allowing early therapeutic intervention.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VERSUS-HOST-DISEASE; MASS-SPECTROMETRY; UNRELATED DONORS; FOLLOW-UP; DIAGNOSIS; GLOBULIN; PREVENTION; BIOMARKERS; LEUKEMIA; CANCER; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 25 Feb 2019 11:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/340 |
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