Schmitt, Eva K. and Vrouenraets, Maarten and Steinem, Claudia (2006) Channel activity of OmpF monitored in nano-BLMs. BIOPHYSICAL JOURNAL, 91 (6). pp. 2163-2171. ISSN 0006-3495,
Full text not available from this repository. (Request a copy)Abstract
Free-standing lipid bilayer membranes can be formed on small apertures (60 nm diameter) on highly ordered porous alumina substrates. The formation process of the membranes on a 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol submonolayer was followed by impedance spectroscopy. After lipid bilayers had thinned, the reconstitution and ionic conducting properties of the outer membrane protein OmpF of E. coli were monitored using single-channel recordings. The characteristic conductance states of the three monomers, fast kinetics, and subconductance states were observed. Blockade of the ion flow as a result of interaction of the antibiotic ampicillin with the protein was verified, indicating the full functionality of the protein channel in nanometer-scale bilayer membranes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BILAYER-LIPID-MEMBRANES; ESCHERICHIA-COLI PORINS; ION-CHANNEL; MOLECULAR-DYNAMICS; BACTERIAL PORINS; PATCH-CLAMP; SILICON; MONOLAYERS; CHIP; RECONSTITUTION; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2021 10:31 |
| Last Modified: | 01 Feb 2021 10:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34053 |
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