Lotter, Kristina and Hoecherl, Klaus and Bucher, Michael and Kees, Frieder (2006) In vivo efficacy of telithromycin on cytokine and nitric oxide formation in lipopolysaccharide-induced acute systemic inflammation in mice. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 58 (3). pp. 615-621. ISSN 0305-7453, 1460-2091
Full text not available from this repository. (Request a copy)Abstract
Objectives: The ketolide telithromycin represents a new subclass of 14-membered semisynthetic macrolides. Because there is evidence that traditional macrolides such as roxithromycin exert anti-inflammatory activity, we investigated the anti-inflammatory action of telithromycin against lipopolysaccharide (LPS)-induced acute systemic inflammation in mice in comparison with roxithromycin. Methods: CD-1 mice were injected intraperitoneally with LPS (1 mg/kg), and the effects of pretreatment with a single intraperitoneal dose of telithromycin (150 mg/kg) or roxithromycin (50 mg/kg) for 2 h on the expression and formation of tumour necrosis factor alpha (TNF alpha), interleukin-1 beta (IL-1 beta), interferon gamma (IFN gamma) and inducible nitric oxide synthase (NOS-II) as well as nitric oxide (NO) were analysed at different time points after LPS-treatment. Cytokine and NOS-II mRNA abundance was examined using real-time RT-PCR. Tissue cytokine levels were determined by enzyme-linked immunosorbent assay kits (ELISA); NO levels were measured by colorimetric assay kits. Results: Pretreatment of mice with telithromycin as well as roxithromycin similarly attenuated the LPS-induced expression and formation of TNF alpha, IL-1 beta and IFN gamma. Furthermore, the LPS-induced increase of NOS-II mRNA and the formation of NO were clearly diminished. Conclusion: These results suggest that the ketolide telithromycin has anti-inflammatory properties like conventional macrolides due to inhibition of the production of proinflammatory cytokines, which leads to a decreased formation of NO in LPS-treated mice. Our data indicate that ketolides may have beneficial therapeutic effects independent of their antibacterial activity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NECROSIS-FACTOR-ALPHA; MACROLIDE ANTIBIOTICS; HUMAN MONOCYTES; SUPPRESSIVE ACTIVITY; KAPPA-B; EXPRESSION; ENDOTOXIN; SYNTHASE; CLARITHROMYCIN; ROXITHROMYCIN; telithromycin; lipopolysaccharide; tumour necrosis factor alpha; interleukin-1 beta; interferon gamma; nitric oxide |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Chemistry and Pharmacy > Institute of Pharmacy > Alumni or Retired Professors > Prof. Frieder Kees |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Feb 2021 06:08 |
| Last Modified: | 03 Feb 2021 06:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34099 |
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