Eble, Johannes A. and Kassner, Anja and Niland, Stephan and Moergelin, Matthias and Grifka, Joachim and Graessel, Susanne (2006) Collagen XVI harbors an integrin alpha 1 beta 1 recognition site in its C-terminal domains. JOURNAL OF BIOLOGICAL CHEMISTRY, 281 (35). pp. 25745-25756. ISSN 0021-9258,
Full text not available from this repository. (Request a copy)Abstract
Collagen XVI is integrated tissue-dependently into distinct fibrillar aggregates, such as D-banded cartilage fibrils and fibrillin-1-containing microfibrils. In skin, the distribution of collagen XVI overlaps that of the collagen-binding integrins alpha 1 beta 1 and alpha 2 beta 1. Basal layer keratinocytes express integrin alpha 2 beta 1, whereas integrin alpha 1 beta 1 occurs in smooth muscle cells surrounding blood vessels, in hair follicles, and on adipocytes. Cells bearing the integrins alpha 1 beta 1 and alpha 2 beta 1 attach and spread on recombinant collagen XVI. Furthermore, collagen XVI induces the recruitment of these integrins into focal adhesion plaques, a principal step in integrin signaling. Of potential physiological relevance, these integrin-collagen XVI interactions may connect cells with specialized fibrils, thus contributing to the organization of fibrillar and cellular components within connective tissues. In cell-free binding assays, collagen XVI is more avidly bound by alpha 1 beta 1 integrin than by alpha 2 beta 1 integrin. Both integrins interact with collagen XVI via the A domain of their alpha subunits. A tryptic collagen XVI fragment comprising the collagenous domains 1 - 3 is recognized by alpha 1 beta 1 integrin. Electron microscopy of complexes of alpha 1 beta 1 integrin with this tryptic collagen XVI fragment or with full-length collagen XVI revealed a unique alpha 1 beta 1 integrin-binding site within collagen XVI located close to its C-terminal end.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-ADHESION; BINDING-SITE; A-DOMAIN; ALPHA(2)BETA(1) INTEGRIN; MONOCLONAL-ANTIBODIES; IV COLLAGEN; ALPHA-2-BETA-1; RECEPTOR; MIGRATION; SKIN; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Feb 2021 06:10 |
| Last Modified: | 03 Feb 2021 06:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34100 |
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