Graf, Marcus and Ludwig, Christine and Kehlenbeck, Sylvia and Jungert, Kerstin and Wagner, Ralf (2006) A quasi-lentiviral green fluorescent protein reporter exhibits nuclear export features of late human immunodeficiency virus type 1 transcripts. VIROLOGY, 352 (2). pp. 295-305. ISSN 0042-6822,
Full text not available from this repository. (Request a copy)Abstract
We have previously shown that Rev-dependent expression of HIV-1 Gag from CMV immediate early promoter critically depends on the AU-rich codon bias of the gag gene. Here, we demonstrate that adaptation of the green fluorescent protein (GFP) reporter gene to HIV codon bias is sufficient to turn this hivGFP RNA into a quasi-lentiviral message following the rules of late lentiviral gene expression. Accordingly, GFP expression was significantly decreased in transfected cells strictly correlating with reduced RNA levels. In the presence of the HIV 5' major splice donor, the hivGFP RNAs were stabilized in the nucleus and efficiently exported to the cytoplasm following fusion of the 3' Rev-responsive element (RRE) and coexpression of HIV-1 Rev. This Rev-dependent translocation was specifically inhibited by leptomycin B suggesting export via the CRM1-dependent pathway used by late lentiviral transcripts. In conclusion, this quasi-lentiviral reporter system may provide a new platform for developing sensitive Rev screening assays. (c) 2006 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REV TRANS-ACTIVATOR; PRE-MESSENGER-RNA; STRUCTURAL GENE-EXPRESSION; HIV REV; INDEPENDENT EXPRESSION; CELLULAR FACTORS; TARGET SEQUENCE; SPLICE SITES; LEPTOMYCIN B; GAG; HIV-1; GFP; codon usage; Rev; RRE; RNA export; CTE; CRM1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Feb 2021 10:19 |
| Last Modified: | 03 Feb 2021 10:19 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34152 |
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