Cabrele, Chiara and Vogel, Mandy and Piso, Pompiliu and Rentsch, Markus and Schroeder, Josef and Jauch, Karl W. and Schlitt, Hans J. and Beham, Alexander (2006) 5-fluorouracil-related enhancement of adenoviral infection is Coxsackievirus-Adenovirus Receptor independent and associated with morphological changes in lipid membranes. WORLD JOURNAL OF GASTROENTEROLOGY, 12 (32). pp. 5168-5174. ISSN 1007-9327,
Full text not available from this repository. (Request a copy)Abstract
AIM: To evaluate the mechanism underlying the effects of 5-Fluorouracil (5-FU) on adenoviral infection. METHODS: Low and high Coxsackievirus-Adenovirus Receptor (CAR) expressing human colon carcinoma cell lines were treated with 5-FU and two El-deleted adenoviral constructs, one transferring GFP (Ad/CMV-GFP) the other bax (Ad/CEA-bax). The number of infected cells were monitored by GFP expression. To evaluate the effects of 5-FU in a receptor free system, Ad/GFP were encapsulated in liposomes and treated with 5-FU. Ad/GFP release was estimated with PCR and infection of 293 cells with the supernatant. Electron microscopy of the Ad5-GFP-liposome complex was made to investigate morphological changes of the liposomes after 5-FU. RESULTS: Infection rates of all cell lines increased from 50% to 98% with emerging 5-FU concentrations. The enhanced viral uptake was independent of the CAR expression. Additionally, 5-FU treated liposomes released 2-2.5 times more adenoviruses. Furthermore, 5-FU-treated liposomes appeared irregular and porous-like. CONCLUSION: adenoviral uptake is enhanced in the presence of 5-FU irrespective of CAR and is associated with morphological changes in membranes making the combination of both a promising option in gene therapy. (C) 2006 The WIG Press. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CANCER CELL-LINES; GENE-TRANSFER; NECK-CANCER; EXPRESSION; CHEMOTHERAPY; CISPLATIN; APOPTOSIS; EFFICACY; ONYX-015; THERAPY; 5-fluorouracil; Coxsackievirus-Adenovirus Receptor; adenoviral infection; gene therapy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Feb 2021 10:46 |
| Last Modified: | 03 Feb 2021 10:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34156 |
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