Belder, Detlev and Kohler, Frank and Ludwig, Martin and Tolba, Kamal and Piehl, Natalia (2006) Coating of powder-blasted channels for high-performance microchip electrophoresis. ELECTROPHORESIS, 27 (16). pp. 3277-3283. ISSN 0173-0835,
Full text not available from this repository. (Request a copy)Abstract
Channels in microfluidic glass chips manufactured with the alternative powder blasting technology were permanently coated with poly(vinyl alcohol) (PVA) in order to improve the performance in microchip electrophoresis. The performance of coated and uncoated powder-blasted (pb) devices as well as coated and uncoated wet chemical etched (wc) chips was compared in electrophoretic separations of fluorescently labeled test compounds. The limited electrophoretic resolution obtained in pb-chips could significantly be improved by coating the channels with PVA. The resolution of test compounds in such coated pb-devices was even higher than in uncoated wc-chips. PVA-coated pb-chips could also successfully be applied in chiral separations. While in an uncoated pb-chip using a cyclodextrins buffer only one broad signal was obtained, two well-resolved signals were obtained in a coated device.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICROFLUIDIC DEVICES; CAPILLARY-ELECTROPHORESIS; FLUORESCENCE DETECTION; SURFACE MODIFICATION; GLASS; SEPARATIONS; TECHNOLOGY; CHIPS; chiral separations; coating; microchip; poly(vinyl alcohol); powder-blasting |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Feb 2021 09:41 |
| Last Modified: | 08 Feb 2021 09:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34198 |
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