Rothhammer, Tanja and Bosserhoff, Anja-Katrin (2006) Influence of melanoma inhibitory activity on transforming growth factor-beta signaling in malignant melanoma. MELANOMA RESEARCH, 16 (4). pp. 309-316. ISSN 0960-8931,
Full text not available from this repository. (Request a copy)Abstract
Melanoma cells escape transforming growth factor-P (TGF beta)-mediated growth inhibition by expressing the Smad (mothers against decapentaplegic homolog, Drosophila) inhibitors Ski and Sno. Here, we demonstrate that melanoma inhibitory activity (MIA) influences the expression of these inhibitors. A Smad responsive reporter construct was activated after TGF beta 1 treatment in the MIA-deficient cell clones but not in the parental cell line. According to this finding, the TGF beta target genes JunB and Id-1 showed a strong induction of expression. Additional analyses revealed that Ski and Sno, repressors of TGF beta/ SMAD signaling, are not expressed in the MIA-deficient cells but in the parental cell line HMB2 and the mock control. Further investigation showed that Ski and Sno expression might be regulated via the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signaling cascade. Treatment of HMB2 cells with a MEK inhibitor revealed a reduction of Ski and Sno expression, which leads to the conclusion that, in our melanoma cell model, Ski and Sno expression is regulated via MAPK/ERK signaling.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ONCOGENIC PROTEIN SKI; MIA; EXPRESSION; CELLS; PROGRESSION; CANCER; MAPK/ERK; melanoma inhibitory activity; Ski; Sno; transforming growth factor-beta signaling |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Feb 2021 11:44 |
| Last Modified: | 09 Feb 2021 11:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34251 |
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