Poppert, Sven and Schlaupitz, Katja and Marre, Reinhard and Voisard, Rainer and Roessler, Wolfgang and Weckermann, Dorothea and Weingaertner, Karl and Essig, Andreas (2006) Chlamydia pneumoniae in an ex vivo human artery culture model. ATHEROSCLEROSIS, 187 (1). pp. 50-56. ISSN 0021-9150,
Full text not available from this repository. (Request a copy)Abstract
The role of the obligate intracellular pathogen Chlamydia pneumoniae in the development of atherosclerosis could not be completely clarified. Reasons are the highly discrepant results obtained in the hitherto existing studies and the lack of an experimental system allowing the direct examination of chlamydial effects in the human vasculature. We established a human ex vivo organ culture model for the characterization of vascular chlamydial infection. Ninety sections of renal arteries, obtained from nephrectomies, were inoculated with Chlamydia pneumoniae. Using a monoclonal FITC-conjugated antibody, chlamydial LPS was broadly detected in inoculated arteries during the entire observation period of 35 days. However, recultivation of viable organisms from the artery vessel wall was impossible, indicating that productive infection in human arteries did not occur even under optimized conditions. This was substantiated by low recovery rates of Chlamydia pneumoniae, low amounts of detectable chlamydial 16S rRNA and ultramorphological presence of polymorph multilamellar bodies in experimentally infected smooth muscle cells originating from aortas, coronary and renal arteries. We could demonstrate that the complex environment of a human artery did not support the growth of Chlamydia pneumoniae although the presence of chlamydial LPS in the artery vessel wall following experimental infection was a common event. The presence of chlamydial LPS in the absence of viable organisms within the artery vessel wall may explain the failure of antibiotic treatment strategies for atherosclerosis. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SMOOTH-MUSCLE-CELLS; CORONARY-HEART-DISEASE; ENDOTHELIAL-CELLS; MYOCARDIAL-INFARCTION; SECONDARY PREVENTION; CYTOKINE PRODUCTION; CONTINUOUS GROWTH; IN-VITRO; ATHEROSCLEROSIS; INFECTION; Chlamydia pneumoniae; atherosclerosis; ex vivo organ culture model; experimental infection; Arteria renalis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Urologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Feb 2021 08:03 |
| Last Modified: | 10 Feb 2021 08:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34327 |
Actions (login required)
 |
View Item |
