Steltenkamp, Siegfried and Mueller, Martin Michael and Deserno, Markus and Hennesthal, Christian and Steinem, Claudia and Janshoff, Andreas (2006) Mechanical properties of pore-spanning lipid bilayers probed by atomic force microscopy. BIOPHYSICAL JOURNAL, 91 (1). pp. 217-226. ISSN 0006-3495, 1542-0086
Full text not available from this repository. (Request a copy)Abstract
We measure the elastic response of a free-standing lipid membrane to a local indentation by using an atomic force microscope. Starting point is a planar gold-coated alumina substrate with a chemisorbed 3-mercaptopropionic acid monolayer displaying circular pores of very well defined and tunable size, over which bilayers composed of N, N,-dimethyl-N,N,dioctadecylammonium bromide or 1,2-dioleoyl-3-trimethylammonium-propane chloride were spread. Centrally indenting these "nanodrums'' with an atomic force microscope tip yields force-indentation curves, which we quantitatively analyze by solving the corresponding shape equations of continuum curvature elasticity. Since the measured response depends in a known way on the system geometry ( pore size, tip radius) and on material parameters ( bending modulus, lateral tension), this opens the possibility to monitor local elastic properties of lipid membranes in a well-controlled setting.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLUID MEMBRANES; POROUS ALUMINA; VESICLES; CELL; SURFACE; SHAPE; ELASTICITY; ADHESION; BIOMEMBRANE; CURVATURE; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Feb 2021 10:39 |
| Last Modified: | 10 Feb 2021 10:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34330 |
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