Klug, Maja and Rehli, Michael (2006) Functional Analysis of Promoter CpG Methylation Using a CpG-Free Luciferase Reporter Vector. EPIGENETICS, 1 (3). pp. 127-130. ISSN 1559-2294, 1559-2308
Full text not available from this repository. (Request a copy)Abstract
Methylation of CpG dinucleotides within proximal promoters is often associated with transcriptional silencing. Methylation-dependent repression is well established for hypermethylated CpG island promoters that are characterized by a high density of CpG residues. The effect of CpG DNA methylation on CpG-poor promoters is less well characterized, probably due to the lack of convenient assay systems to test promoter activities in vitro. In this report, we describe a novel luciferase reporter vector, pCpGL, which completely lacks CpG dinucleotides and can be used to study the effect of promoter DNA methylation in transfection assays. Whereas a traditional reporter vector that contains a large number of backbone CpG residues significantly represses a CpG-free promoter when methylated, our new reporter vector is only repressed due to the presence of functionally important, methylated CpG residues. The pCpGL vector provides a useful tool to study the effects of CpG methylation on CpG-rich and CpG-poor promoters.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; DNA methylation; reporter gene analysis; CpG-free vector; methylation analysis; in vitro methylation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Feb 2021 11:04 |
| Last Modified: | 10 Feb 2021 11:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34338 |
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