Wenke, Ann-Kathrin and Rothhammer, Tanja and Moser, Markus and Bosserhoff, Anja K. (2006) Regulation of integrin alpha 10 expression in chondrocytes by the transcription factors AP-2 epsilon and Ets-1. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 345 (1). pp. 495-501. ISSN 0006-291X, 1090-2104
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Expression of integrin alpha 10 is initiated at the beginning of chondrogenesis and continues throughout cartilage development in adult cartilage. In our study, we aim to identify regulatory sequences that control the cell-type specific expression of the human integrin alpha 10 gene. Therefore, promoter constructs harboring 1139 bp 5' of the transcriptional start site of the human integrin alpha 10 gene were analyzed. Our experiments localized a promoter region that directs high levels of expression specifically in chondrocytes. A sequence analysis detected three consensus AP-2 binding sites within this functional domain. Functionality of these sites was tested and confirmed by cotransfection of AP-2 in a luciferase reporter assay. Interestingly, EMSA identified AP-2 epsilon as the major AP-2 protein binding to the AP-2 consensus sequences. Additionally, Ets-1 was shown to be a positive regulator of the integrin alpha 10 expression whereas Sox9 was irrelevant. Taken together, these results suggest that AP-2 epsilon and Ets-1 are involved in the regulation of integrin 000 transcription in chondrocytes. (c) 2006 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FACTOR AP-2; RETINOIC ACID; MOUSE EMBRYOGENESIS; COLLAGEN GENE; IN-VIVO; BINDING; CLONING; FAMILY; CELLS; SOX9; integrin; cartilage; Chondrocytes; AP-2 epsilon; transcriptional regulator; Ets-1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Feb 2021 12:23 |
| Last Modified: | 11 Feb 2021 12:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34426 |
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