Gorczynski, R.M. and Alexander, C and Bessler, W and Brandenburg, K and Fournier, K and Hoffmann, P and Mach, JP and Mueller, S and Rietschel, ET and Terzioglu, E and Ulmer, AJ and Waelli, T and Zahringer, U and Khatri, I (2006) Role of MIF and glutathione, in association with fetal ovine globin chain (Hb gamma) and LPS, in induction of TNF alpha from cells of young and aged mice, and PBL from healthy human populations. IMMUNOLOGY LETTERS, 105 (2). pp. 140-149. ISSN 0165-2478,
Full text not available from this repository. (Request a copy)Abstract
Previous reports from our group have established that the fetal ovine gamma globin chain (Hb gamma) and LPS can synergize in the induction of pro-inflammatory cytokines, especially TNF alpha, from mouse and human leukocytes. A fetal sheep liver extract (FSLE) which was observed to have marked immunoregulatory properties in vivo and in vitro had independently been observed to contain significant amounts of each of these molecules. However, the biological activity of this extract (hereafter FSLE) was not explained solely by its content of Hb gamma and LPS, and independent analysis confirmed also the presence of migration inhibitory factor, MIF, and glutathione in FSLE. We have investigated whether MIF and the cellular anti-oxidant glutathione can further synergize with Hb gamma and LPS in TNFa induction from human cells in vitro, and mouse cells activated in vivo/in vitro. Our data show that indeed there is evidence for such a synergy. Treatment or mouse cells with FSLE produced an enhanced TNFa production which could be inhibited independently both by anti-Hb gamma and by anti-MIF, and optimally by a combination of these reagents. (c) 2006 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PRODUCTION IN-VIVO; CYTOKINE PRODUCTION; T-CELLS; DENDRITIC CELLS; LIPID-A; HEMOGLOBIN; EXPRESSION; MODULATION; COLITIS; LIPOPOLYSACCHARIDE; cytokine; hemoglobin gamma chain (Hb gamma); migration inhibitory factor (MIF); LPS; TNF alpha |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Feb 2021 12:46 |
| Last Modified: | 11 Feb 2021 12:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34443 |
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