Austin, Bobbie Ann and James, Cassandra M. and Haerle, Peter and Carr, Daniel J. J. (2006) Direct application of plasmid DNA containing type I interferon transgenes to vaginal mucosa inhibits HSV-2 mediated mortality. BIOLOGICAL PROCEDURES ONLINE, 8. pp. 55-62. ISSN 1480-9222,
Full text not available from this repository. (Request a copy)Abstract
The application of naked DNA containing type I interferon (IFN) transgenes is a promising potential therapeutic approach for controlling chronic viral infections. Herein, we detail the application of this approach that has been extensively used to restrain ocular HSV-1 infection, for antagonizing vaginal HSV-2 infection. We show that application of IFN-alpha 1, -alpha 5, and -beta transgenes to vaginal mouse lumen 24 hours prior to HSV-2 infection reduces HSV2 mediated mortality by 2.5 to 3-fold. However, other type I IFN transgenes (IFN- alpha 4, -alpha 5, -alpha 6, and -alpha 9) are non effectual against HSV-2. We further show that the efficacy of IFN-alpha 1 transgene treatment is independent of CD4+ T lymphocytes. However, in mice depleted of CD8+ T lymphocytes, the ability of IFN-alpha 1 transgene treatment to antagonize HSV-2 was lost.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HERPES-SIMPLEX-VIRUS; HUMAN-IMMUNODEFICIENCY-VIRUS; GENITAL HERPES; ENCODING IFN-ALPHA-1; INTRAMUSCULAR INJECTION; HORMONAL CONTRACEPTION; T-LYMPHOCYTES; ALPHA GENE; NAKED DNA; IFN-BETA; virus; interferon; T-lymphocyte; transgene |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Feb 2021 12:48 |
| Last Modified: | 11 Feb 2021 12:48 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34446 |
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