Bataille, Frauke and Troppmann, S and Klebl, F and Rogler, G and Stoelcker, B and Hofstadter, F and Bosserhoff, AK and Rummele, P (2006) Multiparameter immunofluorescence on paraffin-embedded tissue sections. APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, 14 (2). pp. 225-228. ISSN 1062-3345,
Full text not available from this repository. (Request a copy)Abstract
Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin sections has been described previously. The signal intensity observed after triple labeling has been reported to be significantly inferior to that obtained by application of double fluorochromes. The authors show multicolor labeling of three and four primary antibodies in routinely processed paraffin-embedded tissue sections using a standardized immunofluorescence technique. In addition, procedures to reduce background staining and to avoid nonspecific double staining are described.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXPRESSION; MICROSCOPY; TUMORS; immunofluorescence; paraffin; formalin-fixed tissue; multicolor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 06:01 |
| Last Modified: | 15 Feb 2021 06:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34457 |
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