Vollmann, Arabel and Vornlocher, Hans-Peter and Stempfl, Thomas and Brockhoff, Gero and Apfelbaum, Rainer and Bogdahn, Ulrich (2006) Effective silencing of EGFR with RNAi demonstrates non-EGFR dependent proliferation of glioma cells. INTERNATIONAL JOURNAL OF ONCOLOGY, 28 (6). pp. 1531-1542. ISSN 1019-6439,
Full text not available from this repository. (Request a copy)Abstract
The epidermal growth factor receptor (EGFR, ErbB 1) is frequently dysregulated in a variety of solid human tumors, including malignant glioma. EGFR expression has been associated with disease progression, resistance to standard therapies and poor survival. The application of small interfering RNAs (siRNAs) has become an effective and highly specific tool to modulate gene expression, and a wide range of oncogenes have been silenced successfully. Here we show the siRNA-mediated down-regulation of EGFR in two established glioma cell lines with different EGFR expression levels (U373 MG, LN18). The expression of EGFR mRNA and protein was down-regulated by 70-90%. However, siRNA treatment had no inhibitory effect on cell proliferation, migration and activation status of EGFR-coupled signaling cascades. In accordance with these results, gene expression analysis with microarrays revealed only small, albeit specific changes in expression patterns. In conclusion, these data indicate that the specific down-regulation of EGFR might not be sufficient for a single agent therapeutic approach in malignant glioma.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EPIDERMAL-GROWTH-FACTOR; FACTOR RECEPTOR EXPRESSION; TYROSINE KINASE INHIBITOR; MAMMARY EPITHELIAL-CELLS; CULTURED-MAMMALIAN-CELLS; SMALL INTERFERING RNAS; GLIOBLASTOMA-MULTIFORME; MALIGNANT GLIOMAS; MESSENGER-RNA; LUNG-CANCER; AG1478; epidermal growth factor receptor; gene expression analysis; glioma; siRNA |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie Medicine > Lehrstuhl für Pathologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 08:10 |
| Last Modified: | 15 Feb 2021 08:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34494 |
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