Buettner, Roland and Parhofer, K. G. and Woenckhaus, M. and Wrede, C. E. and Kunz-Schughart, Leoni A. and Schoelmerich, Juergen and Bollheimer, L. C. (2006) Defining high-fat-diet rat models: metabolic and molecular effects of different fat types. JOURNAL OF MOLECULAR ENDOCRINOLOGY, 36 (3). pp. 485-501. ISSN 0952-5041
Full text not available from this repository. (Request a copy)Abstract
High-fat (HF)-diet rodent models have contributed significantly to the analysis of the pathophysiology of the insulin resistance syndrome, but their phenotype varies distinctly between different studies. Here, we have systematically compared the metabolic and molecular effects of different HF with varying fatty acid compositions. Male Wistar rats were fed HF diets (42% energy; fat sources: HF-L - lard; HF-O - olive oil; HF-C - coconut fat; HF-F - fish oil). Weight, food intake, whole-body insulin tolerance and plasma parameters of glucose and lipid metabolism were measured during a 12-week diet course. Liver histologies and hepatic gene expression profiles, using Affymetrix GeneChips, were obtained. HF-L and HF-O fed rats showed the most pronounced obesity and insulin resistance; insulin sensitivity in HF-C and HF-F was close to normal. Plasma omega 3 polyunsaturated fatty acid (omega-3-PUFA) and saturated fatty acid (C-12-C-14, SFA) levels were elevated in HF-F and HF-C animals respectively. The liver histologies showed hepatic steatosis in HF-L, HF-O and HF-C without major inflammation. Hepatic SREBP1c-dependent genes were upregulated in these diets, whereas PPAR alpha-dependent genes were predominantly upregulated in HF-F fed rats. We detected classical HF effects only in diets based on lard and olive oil (mainly long-chain, saturated (LC-SFA) and monounsaturated fatty acids (MUFA)). PUFA- or MC-SFA-rich diets did not induce insulin resistance. Diets based on LC-SFA and MUFA induced hepatic steatosis with SREBP1c activation. This points to an intact transcriptional hepatic insulin effect despite resistance to insulin's metabolic actions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEPATIC GENE-EXPRESSION; ELEMENT-BINDING PROTEIN; OIL-FED RATS; NF-KAPPA-B; INSULIN-RESISTANCE; FISH-OIL; INDUCED OBESITY; DIABETES-MELLITUS; GLUCOSE-TOLERANCE; ARACHIDONIC-ACID; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 09:38 |
| Last Modified: | 23 Mar 2022 06:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34504 |
Actions (login required)
 |
View Item |
