Zirak, P. and Penzkofer, A. and Schiereis, T. and Hegemann, P. and Jung, A. and Schlichting, I. (2006) Photodynamics of the small BLUF protein BlrB from Rhodobacter sphaeroides. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 83 (3). pp. 180-194. ISSN 1011-1344,
Full text not available from this repository. (Request a copy)Abstract
The BLUF protein BlrB from the non-sulphur anoxyphototrophic purple bacterium Rhodobacter sphaeroides is characterized by absorption and emission spectroscopy. BlrB expressed from E coli binding FAD, FMN, and riboflavin (called BrlB(I)) and recombinant BlrB containing only FAD (called BlrB(II)) are investigated. The dark-adapted proteins exist in two different receptor conformations (receptor states) with different sub-nanosecond fluorescence lifetimes (BLUFr,f and BLUFr,sl). Some of the flavin-cofactor (ca. 8%) is unbound in thermodynamic equilibrium with the bound cofactor. The two receptor conformations are transformed to putative signalling states (BLUFs,f and BLUFs,sl) of decreased fluorescence efficiency and shortened fluorescence lifetime by blue-light excitation. In the dark at room temperature both signalling states recover back to the initial receptor states with a time constant of about 2 s. Quantum yields of signalling state formation of about 90% for BlrB(II) and about 40% for BlrB(I) were determined by intensity dependent transmission measurements. Extended blue-light excitation causes unbound flavin degradation (formation of lumichrome and lumiflavin-derivatives) and bound cofactor conversion to the semiquinone form. The flavin-semiquinone further reduces and the reduced flavin re-oxidizes back in the dark. A photo-dynamics scheme is presented and relevant quantum efficiencies and time constants are determined. (c) 2006 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INDUCED STRUCTURAL-CHANGES; FLAVIN ADENINE-DINUCLEOTIDE; SYNECHOCYSTIS SP PCC6803; FAD-BINDING DOMAIN; AQUEOUS-SOLUTION; SPECTROSCOPIC CHARACTERIZATION; CHLAMYDOMONAS-REINHARDTII; EUGLENA-GRACILIS; GENE-EXPRESSION; LIGHT RECEPTOR; BLUF domain; BlrB from Rhodobacter sphaeroides; blue-light photoreceptor; absorption spectroscopy; fluorescence spectroscopy; photocycle; photo-degradation; photo-reduction; flavoprotein; flavins; FAD; flavin-semiquinone |
| Subjects: | 500 Science > 530 Physics |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 07:02 |
| Last Modified: | 15 Feb 2021 07:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34509 |
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