Koennings, S. and Garcion, E. and Faisant, N. and Menei, P. and Benoit, J. P. and Goepferich, Achim (2006) In vitro investigation of lipid implants as a controlled release system for interleukin-18. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 314 (2). pp. 145-152. ISSN 0378-5173, 1873-3476
Full text not available from this repository. (Request a copy)Abstract
Operating on the inductive and effective phases of an anti-tumor immune response and uncovering pivotal functions that may reduce cancer cell growth, interleukin-18 (IL-18) appears to be an attractive candidate for the sustained local adjuvant immunotherapeutic treatment of brain gliomas. The objective of this work was to develop IL-18 loaded lipid implants as a controlled delivery system. For the preparation of protein loaded triglyceride matrix material, a solid-in-oil (s/o) dispersion technique was chosen for which protein particles in the micrometer range were first prepared by co-lyophilization with polyethylene glycol (PEG). Implants of 1 mm diameter, 1.8 mm height and 1.8 mg weight were manufactured by compression of the powder mixture in a specially designed powder compacting tool. The in vitro release behavior of I-125-Bolton-Hunter-radiolabeled IL-18 was assessed in a continuous-flow system. A cell culture assay was established for the determination of bioactivity of released IL-18. Implants showed a continuous release of 10-100 ng IL-18 per day for 12 days. A progressive integrity loss was observed with ongoing release, which would be related to protein degradation during incubation. The initially released fraction proved complete retention of bioactivity, indicating that the manufacturing procedure had no detrimental effects on protein stability. (c) 2006 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLY(ETHYLENE GLYCOL); PLGA MICROSPHERES; BIODEGRADABLE MICROSPHERES; DELIVERY-SYSTEMS; PROTEIN; MICROPARTICLES; LYOPHILIZATION; ENCAPSULATION; CELLS; AGGREGATION; lipid implants; protein release; polyethylene glycol; interleukin-18; biological activity assay |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 07:53 |
| Last Modified: | 15 Feb 2021 07:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34538 |
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