Blindt, Ruediger and Vogt, Felix and Astafieva, Irina and Fach, Christian and Hristov, Mihail and Krott, Nicole and Seitz, Berthold and Kapurniotu, Aphrodite and Kwok, Connie and Dewor, Manfred and Bosserhoff, Anja-Katrin and Bernhagen, Juergen and Hanrath, Peter and Hofmann, Rainer and Weber, Christian (2006) A novel drug-eluting stent coated with an integrin-binding cyclic Arg-Gly-Asp peptide inhibits neointimal hyperplasia by recruiting endothelial progenitor cells. JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 47 (9). pp. 1786-1795. ISSN 0735-1097,
Full text not available from this repository. (Request a copy)Abstract
OBJECTIVES Novel stents loaded with an integrin-binding cyclic Arg-Gly-Asp peptide (cRGD) were analyzed for their potential to limit coronary neointima formation and to accelerate endothelialization by attracting endothelial progenitor cells (EPCs). BACKGROUND Re-endothelialization is important for healing after arterial injury. METHODS Effects of cRGD on EPC number, recruitment In flow, and invasion were analyzed in vitro. A durable polymer coating containing 67 mu g cRGD per stent was developed for Guidant Tetra stents. Twelve cRGD-loaded polymer, 12 unloaded polymer, and 12 bare metal stents were deployed in porcine coronary arteries. Quantification of cRGD in peri-stent tissue was established by high-performance liquid chromatography (HPLC) and mass spectrometry (MS). Histomorphometry and immunostaining were performed after 4 and 12 weeks. Recruitment of labeled porcine EPCs was assessed 7 days after intracoronary infusion. RESULTS The cRGD clearly supported the outgrowth, recruitment, and migration of EPCs in vitro. At 4 weeks, there was no difference for mean neointimal area and percent area stenosis in the cRGD-loaded, polymer, or bare metal stent group. At 12 weeks, neointimal area (2.2 +/- 0.3 mm(2)) and percent area stenosis (33 +/- 5%) were significantly reduced compared with polymer stents (3.8 +/- 0.4 mm(2), 54 +/- 6%; p = 0.010) or bare metal stents (3.8 +/- 0.3 mm(2) 53 +/- 3%; p < 0.001). The HPLC/MS confirmed cRGD tissue levels of 1 to 3 mu g/stent at 4 weeks, whereas cRGD was not detectable at 12 weeks. Staining for CD34 and scanning electron microscopy indicated enhanced endothelial coverage on cRGD-loaded stents at 4 weeks associated with a significant Increase in the early recruitment of infused EPCs. CONCLUSIONS Stent coating with cRGD may be useful for reducing in-stent restenosis by accelerating endothelialization.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SMOOTH-MUSCLE-CELLS; CORONARY-ARTERY; SLOW-RELEASE; MOBILIZATION; RESTENOSIS; CHEMOKINES; MIGRATION; INJURY; REENDOTHELIALIZATION; ADHESION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 10:57 |
| Last Modified: | 15 Feb 2021 10:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34549 |
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