Hornung, Matthias and Farkas, Stefan A. and Sattler, Christine and Schlitt, Hans Juergen and Geissler, Edward K. (2006) DX5(+)NKT cells induce the death of colitis-associated cells: involvement of programmed death ligand-1. EUROPEAN JOURNAL OF IMMUNOLOGY, 36 (5). pp. 1210-1221. ISSN 0014-2980,
Full text not available from this repository. (Request a copy)Abstract
NKT cells are activated by CD1d and show an immune regulating function. Here, we investigated whether DX5(+)NKT cells could be used to reduce colitis in a chronic colitis mouse model and studied the potential immunological mechanisms involved. Chronic colitis was induced either by transfer of enriched CD62L(+)CD4(+) T cells to severe-combined-immunodeficient mice or by feeding dextran sodium sulfate to immune competent mice. DX5+NKT cells were transferred to mice with chronic colitis. Co-transfer of DX5(+)NKT cells, but not CD8(+) control cells, prevented the onset of colitis, and the immune regulatory effect of DX5(+)NKT cells was completely abrogated by injecting CD1d blocking antibody. Moreover, DX5(+)NKT cells reduced established colitis in both chronic colitis models. In vitro, DX5(+)NKT cells induced cell death of colon-infiltrating lymphocytes isolated from diseased mice. This effect was inhibited in the presence of either anti-CD1d or anti-programmed death ligand-1 (PD-L1) blocking antibodies. The specific potency of DX5(+)NKT cells in regulating chronic colitis in two mouse models is demonstrated. In vitro testing suggests that DX5(+)NKT cells activated by CD1d induce cell death of colitis-inducing lymphocytes, which is mediated through PD-L1. Therefore, DX5(+)NKT cells could be important in the regulation of immune responses associated with chronic colitis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY-BOWEL-DISEASE; KILLER T-CELLS; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; V(ALPHA)14 NKT CELLS; SCID MICE; ALPHA-GALACTOSYLCERAMIDE; INTESTINAL INFLAMMATION; ANTIGEN PRESENTATION; ADOPTIVE TRANSFER; INNATE IMMUNITY; CD1d; cell transfer; colitis; DX5(+)NKT cells; programmed death ligand-1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 11:43 |
| Last Modified: | 15 Feb 2021 11:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34568 |
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