Palumbo, Emmanuelle and Deghorain, Marie and Cocconcelli, Pier Sandro and Kleerebezem, Michiel and Geyer, Armin and Hartung, Thomas and Morath, Siegfried and Hols, Pascal (2006) D-Alanyl ester depletion of teichoic acids in Lactobacillus plantarum results in a major modification of lipoteichoic acid composition and cell wall perforations at the septum mediated by the Acm2 autolysin. JOURNAL OF BACTERIOLOGY, 188 (10). pp. 3709-3715. ISSN 0021-9193, 1098-5530
Full text not available from this repository. (Request a copy)Abstract
The insertional inactivation of the dlt operon from Lactobacillus plantarum NCIMB8826 had a strong impact on lipoteichoic acid (LTA) composition, resulting in a major reduction in D-alanyl ester content. Unexpectedly, mutant LTA showed high levels of glucosylation and were threefold longer than wild-type LTA. The dlt mutation resulted in a reduced growth rate and increased cell lysis during the exponential and stationary growth phases. Microscopy analysis revealed increased cell length, damaged dividing cells, and perforations of the envelope in the septal region. The observed defects in the separation process, cell envelope perforation, and autolysis of the dlt mutant could be partially attributed to the L. plantarum Acm2 peptidoglycan hydrolase.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ALANINE RACEMASE GENE; LACTOCOCCUS-LACTIS; BACILLUS-SUBTILIS; ANTIMICROBIAL PEPTIDES; NUCLEOTIDE-SEQUENCE; CARRIER PROTEIN; DLT OPERON; BIOSYNTHESIS; CLONING; PEPTIDOGLYCAN; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Anorganische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 12:37 |
| Last Modified: | 15 Feb 2021 12:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34583 |
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