Ecker, M. and Deutzmann, Rainer and Lehle, Ludwig and Mrsa, V. and Tanner, Widmar (2006) Pir proteins of Saccharomyces cerevisiae are attached to beta-1,3-glucan by a new protein-carbohydrate linkage. JOURNAL OF BIOLOGICAL CHEMISTRY, 281 (17). pp. 11523-11529. ISSN 0021-9258, 1083-351X
Full text not available from this repository. (Request a copy)Abstract
A family of covalently linked cell wall proteins of Saccharomyces cerevisiae, called Pir proteins, are characterized by up to 10 conserved repeating units. Ccw5/Pir4p contains only one complete repeating sequence and its deletion caused a release of the protein into the medium. The exchange of each of three glutamines ( Gln(69), Gln(74), Gln(76)) as well as one aspartic acid ( Asp(72)) within the repeating unit leads to a loss of the protein from the cell wall. Amino acid sequencing revealed that only Gln(74) is modified. Release of the protein with mild alkali, changed Gln(74) to glutamic acid, suggesting that Gln(74) is involved in the linkage. Analysis by mass spectrometry showed that 5 hexoses are attached to Gln/Glu(74). Sugar analysis revealed glucose as the only constituent. It is suggested that Pir proteins form novel, alkali labile ester linkages between the gamma-carboxyl group of glutamic acids, arising from specific glutamines, with hydroxyl groups of glucoses of beta-1,3-glucan chains. This transglutaminase-type reaction could take place extracellularly and would energetically proceed on the account of amido group elimination.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | YEAST-CELL WALL; SECRETORY GLYCOPROTEIN; CROSS-LINKING; HEAT-SHOCK; IDENTIFICATION; ARCHITECTURE; BINDING; TRANSGLUTAMINASES; GLYCOSYLATION; MANNOPROTEIN; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Feb 2021 13:51 |
| Last Modified: | 15 Feb 2021 13:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34651 |
Actions (login required)
 |
View Item |
