Castrop, Hayo and Oppermann, M and Weiss, Y and Huang, Y and Mizel, D and Lu, H and Germain, S and Schweda, Frank and Theilig, F and Bachmann, S and Briggs, J and Kurtz, Armin and Schnermann, J (2006) Reporter gene recombination in juxtaglomerular granular and collecting duct cells by human renin promoter-Cre recombinase transgene. PHYSIOLOGICAL GENOMICS, 25 (2). pp. 277-285. ISSN 1094-8341, 1531-2267
Full text not available from this repository. (Request a copy)Abstract
To assess the feasibility of using the renin promoter for expressing Cre recombinase in juxtaglomerular (JG) cells only, we generated five independent transgenic mouse lines (designated hRen-Cre) expressing Cre recombinase under control of a 12.2-kb human renin promoter. In the kidneys of adult mice Cre mRNA (RT-PCR) was found in the renal cortex, with Cre protein (immunohistochemistry) being localized in afferent arterioles and to a lower degree in interlobular arteries. Cre mRNA levels were regulated in a renin-typical fashion by changes in oral salt intake, water restriction, or isoproterenol infusion, indicating the presence of key regulatory elements within 12.2 kb of the 5'-flanking region of the human renin gene. hRen-Cre mice were interbred with both the ROSA26-EGFP and ROSA26-lacZ reporter strains to assess renin promoter activity from Cre-mediated excision of a floxed stop cassette and subsequent enhanced green fluorescent protein (EGFP) and beta-galactosidase (beta-gal) detection. In adult mice, beta-gal staining and EGFP were observed in afferent arterioles and interlobular arteries, overlapping with Cre protein expression. In addition, intense beta-gal staining was found in cortical and medullary collecting ducts where Cre expression was minimal. In embryonic kidneys, beta-gal staining was detected in the developing collecting duct system beginning at embryonic day 12, showing substantial activity of the human renin promoter in the branching ureteric bud. Our data indicate that besides its well-known activity in JG cells and renal vessels the human renin promoter is transiently active in the collecting duct system during kidney development, complicating the use of this approach for JG cell-specific excision of floxed targets.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT HYPERTENSIVE-RATS; ANGIOTENSIN-SYSTEM; MESSENGER-RNA; TUBULAR RENIN; EXPRESSION; MICE; MOUSE; TRANSCRIPTION; KIDNEY; ENHANCEMENT; renin; kidney development |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Frank Schweda Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Wolf Hayo Castrop |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Feb 2021 09:56 |
| Last Modified: | 16 Feb 2021 09:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34694 |
Actions (login required)
 |
View Item |
