Reinhold, Stephan W. and Vitzthum, Helga and Filbeck, T and Wolf, K and Lattas, C and Riegger, Guenther A. J. and Kurtz, Armin and Kraemer, Bernhard K. (2006) Gene expression of 5-, 12-, and 15-lipoxygenases and leukotriene receptors along the rat nephron. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 290 (4). F864-F872. ISSN 1931-857X,
Full text not available from this repository. (Request a copy)Abstract
The arachidonate signaling pathways comprise prostanoids formed by cyclooxygenases, EETs, and HETEs formed by cytochrome P-450 ( CYP) enzymes and HETEs and leukotrienes generated by lipoxygenases. Whereas the intrarenal localization of cyclooxygenases and of some CYP enzymes along the nephron has already been determined, the localization of lipoxygenases and leukotriene-forming enzymes together with leukotriene receptors in the kidney is less clear. This study therefore aimed to determine the expression of 5-, 12-, and 15-lipoxygenases as well as the leukotriene receptors along the rat nephron. The kidneys were dissected into cortex and outer and inner medulla, and the microdissected nephron segments were collected after a collagenase digestion. mRNA abundance was determined by RT-PCR and real-time PCR. 15-LOX mRNA showed a characteristic expression pattern along the distal nephron. 12-LOX mRNA was only found in the glomerulus. Similarly, 5-LOX mRNAs together with 5-LOX-activating protein mRNAs were expressed in the glomerulus and also in the vasa recta. The leukotriene A4 hydrolase was found in all nephron segments, whereas leukotriene C4 synthase mRNA could not be found in any nephron segment. The leukotriene receptor B4 and the cysteinyl leukotriene receptor type 1 were selectively expressed in the glomerulus, whereas cysteinyl receptor type 2 was not found in any nephron segment. Our data suggest that the glomerulus is a major source and target for 5- and 12- HETE and for leukotrienes. The collecting duct system, on the other hand, appears to be a major source of 15-HETE.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYSTEINYL-LEUKOTRIENES; ACTIVATING PROTEIN; MESANGIAL CELLS; ANGIOTENSIN-II; LOCALIZATION; LEUKOCYTES; 12/15-LIPOXYGENASE; VASOCONSTRICTION; BIOSYNTHESIS; INFLAMMATION; nephron segments; microdissection |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Medicine > Lehrstuhl für Urologie Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Feb 2021 10:31 |
| Last Modified: | 16 Feb 2021 10:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34707 |
Actions (login required)
 |
View Item |
