Neumeier, Markus and Weigert, Johanna and Schcaeffler, Andreas and Wehrwein, Gabriele and Mueller-Ladner, Ulf and Schoelmerich, Juergen and Wrede, Christian and Buechler, Christa (2006) Different effects of adiponectin isoforms in human monocytic cells. JOURNAL OF LEUKOCYTE BIOLOGY, 79 (4). pp. 803-808. ISSN 0741-5400,
Full text not available from this repository. (Request a copy)Abstract
Adiponectin (APM) is an adipocyte-derived adipokine with immunosuppressive, antidiabetic, and antiatherosclerotic properties. Low molecular weight (LMW)- and higher molecular weight (HMW)-APM circulate in the serum and activate different signaling pathways. We were interested to see whether LMW-APM exerts different effects on monocytic cells compared with the HMW isoform. Therefore, the effects of recombinant LMW-APM produced in insect cells and the APM from higher eukaryotic cells containing HMW forms on monocytic cells were investigated with respect to apoptosis and inflammation. LMW- and HMW-APM induce apoptosis in non-differentiated THP-1 cells, reduce macrophage scavenger receptor (MSR) A mRNA expression, and stimulate phosphorylation of adenosine nionophosphate-activated protein kinase (AMPK). However, HMW-APM induces the secretion of interleukin (IL)-6 in human monocytes and THP-1 cells but does not suppress lipopolysaccharide (LPS)-induced IL-6 secretion. In contrast, LMW-APM reduces LPS-mediated IL-6 release and furthermore, stimulates IL-10 secretion, most likely by reducing the abundance of inhibitor of nuclear factor (NF)-kappa B kinase beta, leading to a diminished nuclear translocation of NF-kappa B p65. Our data indicate that the different APM isoform. s do share common effects on monocytic ceRs but also induce isoform-specific responses. Although apoptosis, the activation of AMPK, and the reduction of MSR are mediated by all APM isoforms, only LMW-APM displays anti-inflammatory properties.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED PROTEIN-KINASE; LYSOSOMAL ACID LIPASE; HUMAN BLOOD MONOCYTES; FACTOR-KAPPA-B; HUMAN MACROPHAGES; ADIPOSE-TISSUE; DEFICIENT MICE; TNF-ALPHA; ATHEROSCLEROSIS; EXPRESSION; endotoxin; inflammation; adipokine; diabetes |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Feb 2021 09:08 |
| Last Modified: | 17 Feb 2021 09:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34746 |
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