Glockzin, Gabriel and Mantwill, Klaus and Jurchott, Karsten and Bernshausen, Alexandra and Ladhoff, Axel and Royer, Hans-Dieter and Gansbacherf, Bernd and Holm, Per Sonne (2006) Characterization of the recombinant adenovirus vector AdYB-1: Implications for oncolytic vector development. JOURNAL OF VIROLOGY, 80 (8). pp. 3904-3911. ISSN 0022-538X, 1098-5514
Full text not available from this repository. (Request a copy)Abstract
Conditionally replicating adenoviruses are a promising new modality for the treatment of cancer. However, early clinical trials demonstrate that the efficacy of current vectors is limited. Interestingly, DNA replication and production of viral particles do not always correlate with virus-mediated cell lysis and virus release depending on the vector utilized for infection. However, we have previously reported that nuclear accumulation of the human transcription factor YB-1 by regulating the adenoviral E2 late promoter facilitates viral DNA replication of Et-deleted adenovirus vectors which are widely used for cancer gene therapy. Here we report the promotion of virus-mediated cell killing as a new function of the human transcription factor YB-1. In contrast to the E1A-deleted vector dl312 the first-generation adenovirus vector AdYB-1, which overexpresses YB-1 under cytomegalovirus promoter control, led to necrosis-like cell death, virus production, and viral release after infection of A549 and U2OS tumor cell lines. Our data suggest that the integration of YB-1 in oncolytic adenoviruses is a promising strategy for developing oncolytic vectors with enhanced potency against different malignancies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BOX-BINDING PROTEIN-1; P53 TUMOR-SUPPRESSOR; LATE GENE-EXPRESSION; EARLY REGION 4; TRANSCRIPTION FACTOR; NUCLEAR EXPRESSION; DNA-REPLICATION; P53-INDEPENDENT APOPTOSIS; P-GLYCOPROTEIN; INFECTED-CELLS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 17 Feb 2021 09:46 |
| Last Modified: | 17 Feb 2021 09:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34755 |
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