Lee, Bong-Seop and Fujita, M and Khazenzon, NM and Wawrowsky, KA and Wachsmann-Hogiu, S and Farkas, DL and Black, KL and Ljubimova, JY and Holler, Eggehard (2006) Polycefin, a new prototype of a multifunctional nonconjugate based on poly(beta-L-malic acid) for drug delivery. BIOCONJUGATE CHEMISTRY, 17 (2). pp. 317-326. ISSN 1043-1802,
Full text not available from this repository. (Request a copy)Abstract
A new prototype of nanoconjugate, Polycefin, was synthesized for targeted delivery of antisense oligonucleotides and monoclonal antibodies to brain tumors. The macromolecular carrier contains: 1. biodegradable, nonimmunogenic, nontoxic beta-poly(L-malic acid) of microbial origin; 2. Morpholino antisense oligonucleotides targeting laminin alpha 4 and beta 1 chains of laminin-8, which is specifically overexpressed in glial brain tumors; 3. monoclonal anti-transferrin receptor antibody for specific tissue targeting; 4. oligonucleotide releasing disulfide units; 5. L-valine containing, pH-sensitive membrane disrupting unit(s), 6. protective poly(ethylene glycol); 7. a fluorescent dye (optional). Highly purified modules were conjugated directly with N-hydroxysuccinimidyl ester-activated beta-poly(L-Malic acid) at pendant carboxyl groups or at thiol containing spacers via thioether and disuffide bonds. Products were chemically validated by physical, chemical, and functional tests. In vitro experiments using two human glioma cell lines U87MG and T98G demonstrated that Polycefin was delivered into the tumor cells by a receptor-mediated endocytosis mechanism and was able to inhibit the synthesis of laminin-8 alpha 4 and beta 1 chains at the same time. Inhibition of laminin-8 expression was in agreement with the designed endosomal membrane disruption and drug releasing activity. In vivo imaging showed the accumulation of intravenously injected Polycefin in brain tumor tissue via the anti body-targeted transferrin receptor-mediated endosomal pathway in addition to a less efficient mechanism known for high molecular mass biopolymers as enhanced permeability and retention effect. Polycefin was nontoxic to normal and tumor astrocytes in a wide range of concentrations, accumulated in brain tumor, and could be used for specific targeting of several biomarkers simultaneously.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLOOD-BRAIN-BARRIER; TRANSFERRIN RECEPTOR; CANCER-CHEMOTHERAPY; HPMA COPOLYMER; IN-VITRO; MACROMOLECULAR THERAPEUTICS; ANTICANCER DRUGS; CELL-LINES; POLYMER; DESIGN; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Alumni or Retired > Prof. Dr. Eggehard Holler |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Feb 2021 09:36 |
| Last Modified: | 18 Feb 2021 09:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34806 |
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