Hoffmann, Petra and Boeld, TJ and Eder, R and Albrecht, J and Doser, K and Pieshka, B and Dada, A and Niemand, C and Assenmacher, M and Orso, E and Andreesen, Reinhard and Holler, Ernst and Edinger, Matthias (2006) Isolation of CD4(+)CD25(+) regulatory T cells for clinical trials. BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, 12 (3). pp. 267-274. ISSN 1083-8791, 1523-6536
Full text not available from this repository. (Request a copy)Abstract
The adoptive transfer of donor CD4(+)CD25(+) regulatory T cells has been shown to protect from lethal graft-versus-host disease after allogeneic bone marrow transplantation in murine disease models. Efficient isolation strategies that comply with good manufacturing practice (GMP) guidelines are prerequisites for the clinical application of human CD4(+)CD25(+) regulators, T cells. Here we describe the isolation of CD4(+)CD25(+) T cells with regulatory function from standard leukapheresis products by using a 2-step magnetic cell-separation protocol performed under GMP conditions. The generated cell products contained on average 49.5% CD4(+)CD25(high) T cells that phenotypically and functionally represented natural CD4(+)CD25(+) regulatory T cells and showed a suppressive activity comparable to that of CD4(+)CD25(+) regulatory T-cell preparations purified by non-GMP-approved fluorescence-activated cell sorting. (C) 2006 American Society for Blood and Marrow Transplantation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VERSUS-HOST-DISEASE; BONE-MARROW-TRANSPLANTATION; IN-VITRO; FOXP3; SUPPRESSION; POPULATION; INFUSION; LEUKEMIA; BLOOD; anergy; tolerance; suppressor T lymphocytes; graft-versus-host disease; good manufacturing practice |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Feb 2021 09:38 |
| Last Modified: | 18 Feb 2021 09:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34807 |
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