Spagnuolo, G. and D'Anto, V. and Cosentino, C. and Schmalz, Gottfried and Schweikl, Helmut and Rengo, S. (2006) Effect of N-acetyl-L-cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts. BIOMATERIALS, 27 (9). pp. 1803-1809. ISSN 0142-9612, 1878-5905
Full text not available from this repository. (Request a copy)Abstract
Previous investigations have shown that 2-hydroxyethyl methacrylate (HEMA) causes reactive oxygen species (ROS) production, which in turn affects cell survival and cell death. The purpose of this study was to evaluate the effects of the antioxidant N-acetyl-L-cysteine (NAC) on HEMA-induced toxicity in human primary gingival fibroblasts (HGF). HGF were treated with various concentrations of HEMA (0-12 mm) in the absence and presence of NAC (1, 5, and 10 mm). The 3-(4,5 dimethyiazol-2-1)-2-5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the mitochondrial dehydrogenase activity after HEMA exposure. Viability and cell death were determined by flow cytometry using Annexin V and PI staining. ROS production was detected by the increasing fluorescence of the oxidation-sensitive dye 2',7'-dichlorofluorescein diacetate (DCFH-DA) after HEMA treatment. After a 24 h incubation period, HEMA concentrations higher then 10 mM caused a decrease of cell viability, mitochondrial activity, and ail increase of cell death. HEMA concentrations of 4-12 mm markedly increased ROS levels in a dose-dependent manner. High NAC concentrations (5 and 10 mm) significantly reduced cell death, and restored the mitochondrial activity after a 24 h co-treatment, but 1 mm NAC increased HEMA toxicity (p < 0.05). All NAC concentrations significantly reduced ROS levels induced by HEMA after a 2 It exposure (P < 0.05), but no such reduction was observed after a 4 h treatment. Furthermore, treatment with 10 mM HEMA and 1 mm NAC for 6 h caused an increase in ROS levels compared to 10mm HEMA alone (p<0.05). In conclusion. Our results suggest that high NAC concentrations protect HGF against HEMA cytotoxicity by reducing the induced ROS levels. (C) 2005 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OXIDATIVE STRESS; IN-VITRO; GLUTATHIONE DEPLETION; FILLING MATERIALS; EPITHELIAL-CELLS; FREE-RADICALS; CYCLE ARREST; PULP CELLS; CYTOTOXICITY; APOPTOSIS; dental resin; HEMA; human gingival fibroblasts; NAC; reactive oxygen species |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Feb 2021 09:42 |
| Last Modified: | 18 Feb 2021 09:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34809 |
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