Voeller, Daniel and Linck, Lisa and Bruckmann, Astrid and Hauptmann, Judith and Deutzmann, Rainer and Meister, Gunter and Bosserhoff, Anja Katrin (2016) Argonaute Family Protein Expression in Normal Tissue and Cancer Entities. PLOS ONE, 11 (8): e0161165. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Themembers of the Argonaute (AGO) protein family are key players inmiRNA-guided gene silencing. They enable the interaction between small RNAs and their respective target mRNA (s) and support the catalytic destruction of the gene transcript or recruit additional proteins for downstream gene silencing. The human AGO family consists of four AGO proteins (AGO1-AGO4), but only AGO2 harbors nuclease activity. In this study, we characterized the expression of the four AGO proteins in cancer cell lines and normal tissues with a new mass spectrometry approach called AGO-APP (AGO Affinity Purification by Peptides). In all analyzed normal tissues, AGO1 and AGO2 were most prominent, but marked tissue-specific differences were identified. Furthermore, considerable changes during development were observed by comparing fetal and adult tissues. We also identified decreased overall AGO expression in melanoma derived cell lines compared to other tumor cell lines and normal tissues, with the largest differences in AGO2 expression. The experiments described in this study suggest that reduced amounts of AGO proteins, as key players in miRNA processing, have impact on several cellular processes. Deregulated miRNA expression has been attributed to chromosomal aberrations, promoter regulation and it is known to have a major impact on tumor development and progression. Our findings will further increase our basic understanding of the molecular basis ofmiRNA processing and its relevance for disease.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MALIGNANT-MELANOMA; MICRORNA EXPRESSION; TARGET GENES; DICER; PROGRESSION; MIRNA; COMPLEXES; RNA; BIOGENESIS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2019 08:31 |
| Last Modified: | 03 Apr 2019 08:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3484 |
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