Simbuerger, Johann M. B. and Dettmer, Katja and Oefner, Peter J. and Reinders, Joerg (2016) Optimizing the SWATH-MS-workflow for label-free proteomics. JOURNAL OF PROTEOMICS, 145. pp. 137-140. ISSN 1874-3919, 1876-7737
Full text not available from this repository. (Request a copy)Abstract
Quantification of proteins by mass spectrometry at the level of entire proteomes has become a central aspect in contemporary proteomics. Sequential Window Acquisition of all THeoretical fragment-ion spectra (SWATH) mass spectrometry is a label-free protein quantification method enabling parallel quantification of up to several thousand proteins, thereby granting a comprehensive overview of highly complex samples. We optimized several parameters of the mass spectrometrical detection to increase the size of the library, enhance the precision of protein quantifications and decrease systematic errors induced by fragment ion interference. By these means enhanced differential analyses facilitate the detection of more significantly regulated proteins with a dynamic range of over three orders of magnitude. (C) 2016 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MASS-SPECTROMETRY; SWATH; Mass spectrometry; Label free quantification |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2019 08:35 |
| Last Modified: | 03 Apr 2019 08:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3486 |
Actions (login required)
 |
View Item |
