Kruppa, Michael and Frank, Daniel and Leffler-Schuster, Helga and Koenig, Burkhard (2006) Screening of metal complex-amino acid side chain interactions by potentiometric titration. INORGANICA CHIMICA ACTA, 359 (4). pp. 1159-1168. ISSN 0020-1693, 1873-3255
Full text not available from this repository. (Request a copy)Abstract
Reversible coordination of amino acid side chains to metal complexes is widely used in protein purification (IMAC technique), but available data on affinity and selectivity of such binding processes are limited. We use potentiometric titration of a series of metal complexes with vacant coordination sites in the presence of molecules resembling amino acid side chain functionalities to screen for new affinities. The investigation confirms documented affinities of imidazole to nickel(II) and copper(II) IDA and NTA complexes, and discovers a hitherto unknown binding of zinc(II)- and cadmium(II) cyclen complexes to imidazole. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AFFINITY-CHROMATOGRAPHY; SOLUTION CHEMISTRY; CRYSTAL-STRUCTURES; BINDING-SITE; ION; CYCLEN; SYSTEM; potentiometric titration; coordinative binding; IDA; NTA; cyclen; binding constant |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Feb 2021 11:47 |
| Last Modified: | 18 Feb 2021 11:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34860 |
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