Fuchshofer, Rudolf and Welge-Lussen, U. and Lutyen-Drecoll, E. and Birke, Marco (2006) Biochemical and morphological analysis of basement membrane component expression in corneoscleral and cribriform human trabecular meshwork cells. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 47 (3). pp. 794-801. ISSN 0146-0404, 1552-5783
Full text not available from this repository. (Request a copy)Abstract
PURPOSE. To determine whether cells of the cribriform trabecular meshwork (TM) express basement membrane (BM) components similar to corneoscleral TM cells and to determine whether cribriform cells are connected to the elastic tendon net of the TM. METHODS. TM cells of the corneoscleral and the cribriform regions were cultured from 10 eyes of 10 donors, aged 20 to 87 years. Cell types were classified by a-smooth muscle actin (smA), desmin, and all-crystallin staining. Expression of collagen type IV (ColIV) chains alpha 1 to 6; Collagen type VIII (ColVIII) alpha 1; laminin subunits alpha 1 to 5, beta 1 to 3, and gamma 1 to 3; and nidogen 1 and 2 was tested in both cell types by semiquantitative RT-PCR (sqPCR). Expression of ColIV alpha 2, ColVIII alpha 1, laminin beta 2, and nidogen 1 was quantified by Northern blot analysis. The response to transforming growth factor (TGF)-beta 2 treatment was investigated. Serial tangential and sagittal TM sections of 16 eyes from 10 donors (aged 12-90 years) were used for electron- and immunoelectron microscopy. RESULTS. Both TM cell types expressed ColIV chains alpha 1, alpha 2, alpha 4, alpha 5, and alpha 6; ColVIII alpha 1; laminin subunits alpha 3, alpha 4, beta 1, beta 2, beta 3, gamma 1, and gamma 2; and niclogen 1, as determined by Northern blot analysis and sqPCR. ColIV alpha 3; laminin subunits alpha 1, alpha 2, and gamma 3; and nidogen 2 were not detectable by PCR. Responses to TGF-beta 2 treatment did not differ between cell types. In vivo, all cribriform cells were in contact with ColIV containing BM material and were found to connect to the cribriform elastic network. CONCLUSIONS. Cribriform and corneoscleral TM cells show no differences in expression of BM components and response to TGF-beta 2. The direct connection of cribriform cells to the elastic tendon network suggests that they are under mechanical tension. This could explain previous findings of all-crystallin expression in the cribriform, region.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ALPHA-B-CRYSTALLIN; COLLAGEN TYPE-IV; ALPORT-SYNDROME; DESCEMETS-MEMBRANE; VIII COLLAGEN; TGF-BETA-2; LAMININ; TISSUE; CHAINS; GENE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Feb 2021 13:24 |
| Last Modified: | 18 Feb 2021 13:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34867 |
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