Schneider, Tim and Hung, Lee-Hsueh and Schreiner, Silke and Starke, Stefan and Eckhof, Heinrich and Rossbach, Oliver and Reich, Stefan and Medenbach, Jan and Bindereif, Albrecht (2016) CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs. SCIENTIFIC REPORTS, 6: 31313. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Circular RNAs (circRNAs) constitute a new class of noncoding RNAs in higher eukaryotes generated from pre-mRNAs by alternative splicing. Here we investigated in mammalian cells the association of circRNAs with proteins. Using glycerol gradient centrifugation, we characterized in cell lysates circRNA-protein complexes (circRNPs) of distinct sizes. By polysome-gradient fractionation we found no evidence for efficient translation of a set of abundant circRNAs in HeLa cells. To identify circRNPs with a specific protein component, we focused on IMP3 (IGF2BP3, insulin-like growth factor 2 binding protein 3), a known tumor marker and RNA-binding protein. Combining RNA-seq analysis of IMP3-coimmunoprecipitated RNA and filtering for circular-junction reads identified a set of IMP3-associated circRNAs, which were validated and characterized. In sum, our data suggest that specific circRNP families exist defined by a common protein component. In addition, this provides a general approach to identify circRNPs with a given protein component.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-BINDING-PROTEIN; CIRCULAR RNAS; EXON CIRCULARIZATION; BIOGENESIS; REVEALS; IDENTIFICATION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Apr 2019 06:06 |
| Last Modified: | 08 Apr 2019 06:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/3489 |
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