Gruber, Michael and Wiesner, Gunther and Burger, Ralf and Lindner, Regina (2006) The salicylate trapping method: Is oxidation of salicylic acid solution oxygen and time dependent and metal catalysed? JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 831 (1-2). pp. 320-323. ISSN 1570-0232,
Full text not available from this repository. (Request a copy)Abstract
For a microdialytic trapping method we systematically investigated changes in concentrations of 2,5-dihydroxy-benzoic acid (2,5-DHBA) and 2,3-dihydroxy-benzoic acid (2,3-DHBA) in freshly prepared solutions of salicylic acid (SA). The solvent was 0.9% saline exposed to different atmospheric concentrations of oxygen (0, 21, and 100%). The solutions were treated by freezing-thawing and an ultrasonic bath in presence and absence of aluminium foil. Without aluminium the concentrations of 2,5-DHBA and 2,3-DHBA kept constant over an observed period of 160 min on different levels from below 20 ng/ml to about 100 ng/ml. In presence of aluminium the concentrations increased to maximum 307 ng/ml after 160 min. Ultrasonic irradiation amplified this effect to maximum 341 ng/ml. HPLC/ECD processing and quantitative analysis of dihydroxy-benzoic acids (DHBAs) in microdialysis may be artificially influenced by varying oxygen environment and metal catalysis. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HYDROXYL RADICAL GENERATION; TRAUMATIC BRAIN-INJURY; EPIDURAL MASS LESION; ELECTROCHEMICAL DETECTION; LIQUID-CHROMATOGRAPHY; REPRODUCIBLE MODEL; LIPID-PEROXIDATION; RAT STRIATUM; MICRODIALYSIS; METABOLISM; salicylate trapping method; 2,5-DHBA; 2,3-DHBA; hyperoxia; oxygen; metal catalysis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Neurochirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Feb 2021 12:21 |
| Last Modified: | 23 Feb 2021 12:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/34949 |
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