Duerkop, Axel and Turel, Matejka and Lobnik, Aleksandra and Wolfbeis, Otto S. (2006) Microtiter plate assay for phosphate using a europium-tetracycline complex as a sensitive luminescent probe. ANALYTICA CHIMICA ACTA, 555 (2). pp. 292-298. ISSN 0003-2670, 1873-4324
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A new luminescent europium probe is presented for the determination of phosphate (P) in microliter plate format. The assay is based on the quenching of the luminescence of the europium-tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 mu mol/L. The probe is excited at 400 nm and displays a large Stokes' shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 x 10(-6) mol/L to 7.5 x 10(-4) mol/L of phosphate, and the limit of detection is 3 mu mol/L. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | THIN-FILM ELECTRODE; HYDROGEN-PEROXIDE; FLOW-INJECTION; NATURAL-WATER; ION; THIAMINE; phosphate; microplate assay; luminescence; lanthanide |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Feb 2021 11:24 |
| Last Modified: | 24 Feb 2021 11:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35033 |
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