Zhi, Zheng-liang and Drazan, Viktor and Wolfbeis, Otto S. and Mirsky, Vladimir M. (2006) Electrocatalytic activity of DNA on electrodes as an indication of hybridisation. BIOELECTROCHEMISTRY, 68 (1). pp. 1-6. ISSN 1567-5394, 1878-562X
Full text not available from this repository. (Request a copy)Abstract
Electron transfer between metal electrodes and ferro/ferricyanide is completely suppressed at low ionic concentration. We describe here a new phenomenon related to this reaction: an immobilisation of thiolated single-stranded DNA on gold electrodes retains this activity at low ionic strength up to the level corresponding to the high ionic strength. In contrast, a hybridisation of the complementary DNA with the thiolated single stranded DNA followed by a binding onto the electrodes, attenuated the electrocatalytic effect. These effects can be used for discrimination between single-stranded DNA and double-stranded DNA and for semi-quantitative measurement of complementary DNA in a sample. (c) 2005 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMPEDANCE SPECTROSCOPY; SPECIAL ISSUE; OLIGONUCLEOTIDE; BIOSENSOR; SURFACE; SENSOR; ELECTROCHEMISTRY; IMMOBILIZATION; TRANSDUCTION; MOLECULES; DNA; DNA-sensor; biosensor; electrocatalysis; hybridisation; olygonucleotide; gene analysis |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2021 09:16 |
| Last Modified: | 01 Mar 2021 09:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35080 |
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