Boeck, Stefan and Hamann, M. and Pihusch, V. and Heller, T. and Diem, H. and Rolf, B. and Pihusch, R. and Kolb, H. J. and Pihusch, M. (2006) Kinetics of dendritic cell chimerism and T cell chimerism in allogeneic hematopoietic stem cell recipients. BONE MARROW TRANSPLANTATION, 37 (1). pp. 57-64. ISSN 0268-3369,
Full text not available from this repository. (Request a copy)Abstract
Dendritic cells (DC) as potent antigen-presenting cells (APC) and T cells as effector cells play an essential role in the pathophysiology of both graft-versus-host (GvH) and graft-versus-leukemia (GvL) reactions after transplantation. Therefore, we determined the kinetics of DC and T-cell chimerism establishment after allogeneic hematopoietic cell transplantation (AHCT) in a group of 144 patients, using fluorescence-activated cell sorting (FACS) or magnetic cell sorting (MACS) followed by FISH or STR-PCR analysis for chimerism evaluation. In all, three cell lines investigated (CD3+ T cells, CD11c+ DC1 and CD123+ DC2), we found a rapid and consistent establishment of complete donor chimerism (CDC) in over 70% of all patients during the first 6 weeks after AHCT. The rate of patients with CDC increased significantly over time within the first year after transplantation. A related donor (P = 0.004) as well as an underlying lymphatic leukemia (P = 0.03) were found to be significantly associated with development of MC in T cells. No significant correlation between DC or T cell chimerism and GvHD or relapse was detected. Our results thus demonstrate a fast and stable CDC in DC1, DC2 and T cells after AHCT that continuously increases over time in nearly all patients.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VERSUS-HOST-DISEASE; BONE-MARROW-TRANSPLANTATION; TANDEM REPEAT-MARKERS; MIXED CHIMERISM; LANGERHANS CELLS; CHRONIC GRAFT; LEUKEMIA; ORIGIN; PCR; AMPLIFICATION; chimerism; dendritic cells; T cells; graft-versus-host disease |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Mar 2021 09:23 |
| Last Modified: | 01 Mar 2021 09:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/35084 |
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